The Premier Division clubs join in round 4, and the Division One clubs join in the third round.
Last season’s Division three east winners Penygraig play hosts to Brynithel, while Division three west champions take on Felinfoel have a bye, along with 105 clubs who will join in round 2.
Among the upsets could be Cwmgors in their away trip to Seven Sisters v Cwmgors, while Trefil against Hirwaun and Wattstown home to Pontllanfraith could see exciting matches where the league sides are pushed to the limit.
Abertillery hope to raise a side to meet Cardiff Medicals, while the
Toughest clash could be between Corus PT and Aberavon Green Stars with Llantrisant against Bargoed, Nant Conwy home to Abercynon and a much-improved Newport HSOB against Cardigan should be among the highlights.
Five played on North Wales grounds and several involving second-class clubs, that include Welsh District cup winners Clwb Rygbi Caerdydd, who meet league opponents in New Dock Stars on a Cardiff parks pitch.
The final takes place at the Millennium Stadium on May 8. Last years final between Newport and Llanelli saw the Scarlets lift the trophy with a 32-9 victory in front of a crowd of 27,500.
WRU Challenge Cup – Round One
Abercrave v Wrexham
Abertillery v Cardiff Meds
Aberystwyth v Tenby Utd
Aberystwyth Univ v Gwernyfed
Alltwen v Birchgrove
Bangor Normal Coll v Nelson
Bangor Univ v Brecon
Barry v Haverfordwest
Briton Ferry v Monmouth
Bryncoch v Pontyclun
Burry Port v Tycroes
Bynea v Milford Haven
Cardiff Univ v Felinfoel
Clwb Rygbi Caerdydd v New Dock Stars
Corus PT v Aberavon G Stars
Crumlin v Tonna
Cwmavon v Bridgend Sports
Cwmgwrach v Cefn Coed
Cwmllynfell v Loughor
Cwmtwrch v Llantwit Major
Dunvant v Glynneath
Ferndale v Llangefni
Fishguard v Brynamman
Garndiffaith v Gorseinon
Glais v Maesteg Qns
Hendy v Llantwit Fardre
Heol y Cyw v Welshpool
Kenfig Hill v Penygroes
Lampeter Coll v Canton
Llandaff v UWIN
Llanishen v Tonyrefail
Llantrisant v Bargoed
Machen v Bala
Maesteg Celtic v Treherbert
Mumbles v Ynsybwl
Nant Conwy v Abercynon
Newport HSOB v Cardigan
Ogmore Vale v Cowbridge
Old Illtydians v Rhiwbina
Old Penarthians v St Albans
Pembroke Dock Qns v Resolven
Penygraig v Brynithel
Pontycymmer v Mold
Pontypool Utd v BP Llandarcy
Pwllheli v Carmarthen Coll
Pyle v Lampeter
Rhos v Pontyates
Rhyl v Caernarfon
Senghenydd v Cardiff HSOB
Seven Sisters v Cwmgors
Skewen v Crynant
Trefil v Hirwaun
Wattstown v Pontllanfraith
